In vivo diagnosis with purified tropomyosin in mite and shellfish allergic patients

Publication date: Available online 28 April 2016
Source:Annals of Allergy, Asthma & Immunology
Author(s): M. Angeles López-Matas, Carlos H. de Larramendi, Raquel Moya, Inmaculada Sánchez-Guerrero, Angel Ferrer, Angel Julio Huertas, Isabel Flores, Luis Angel Navarro, José Luis García-Abujeta, Sandra Vicario, Carmen Andreu, Maribel Peña, Jerónimo Carnés
BackgroundTropomyosin is the most studied shellfish allergen and has been involved in cross-reactivity among different invertebrates (crustacean, mollusks, mites, insects, and nematodes).ObjectiveTo determine the relevance of tropomyosin in mite- and shellfish-sensitized patients using tropomyosin skin testing.MethodsPatients were divided into 3 groups: group M included mite allergic patients (ie, individuals with respiratory symptoms and a positive result on skin prick testing [SPT] to house dust mites), group S included shellfish allergic patients (ie, individuals who reported symptoms with shellfish), and group MS included mite- and shellfish allergic patients (ie, individuals who simultaneously fulfilled the inclusion criteria for groups M and S). Tropomyosin was purified from shrimp, characterized, and used in SPT for diagnosis in the patient population.ResultsEight hundred fifty patients were included in the study: 790 (92.9%) in group M, 21 (2.5%) in group S, and 39 (4.6%) in group MS. Tropomyosin was purified from shrimp with a purity higher than 95%. Forty-two individuals tested positive to tropomyosin: the prevalence was 2.7% in group M, 28.6% in group S, and 38.5% in patients of group MS. Twenty-one (50%) of the tropomyosin-positive individuals had symptoms with shellfish, and 3 (14.3%) reported anaphylaxis.ConclusionThe prevalence of tropomyosin was low in mite-sensitized patients (2.7 %) and high in shellfish allergic patients (28.6%). The higher prevalence of tropomyosin was found in patients sensitized to both mite and shellfish (38.5%). The selection of tropomyosin-sensitized patients by SPT might help in the choice of appropriate treatments or management for these patients.



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