Interleukin-17A expression in human synovial mast cells in rheumatoid arthritis and osteoarthritis

Publication date: Available online 18 May 2016
Source:Allergology International
Author(s): Jun-ichiro Kan, Shintaro Mishima, Jun-ichi Kashiwakura, Tomomi Sasaki-Sakamoto, Masayuki Seki, Shu Saito, Chisei Ra, Yasuaki Tokuhashi, Yoshimichi Okayama
BackgroundInterleukin (IL)-17A plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA). The expression of IL-17A in synovial mast cells (MCs) in RA and osteoarthritis (OA) has been reported, but the frequencies of IL-17A expression in synovial MCs have varied. The aim of this study was to investigate whether IL-17A expression is upregulated in human synovial MCs in RA and to elucidate the mechanism of IL-17A expression in synovial MCs.MethodsSynovial tissues were obtained from patients with RA or OA undergoing joint replacement surgery, and synovial MCs were enzymatically dispersed. Synovium-derived cultured MCs were generated by culturing synovial cells with stem cell factor. IL-17A expression was investigated using immunofluorescence in synovial tissues. IL-17A mRNA expression and its production from MCs were examined using RT-PCR and ELISA, respectively.ResultsThe number of IL-17A-positive ⁽⁺⁾ synovial MCs and the percentage of IL-17A⁺ MCs among all the IL-17A⁺ cells from RA patients were not significantly increased compared with those from OA subjects. The synovium-derived cultured MCs spontaneously released small amounts of IL-17A. Neither IgE- nor IgG-dependent stimulation increased IL-17A production from the MCs. IL-33, tumor necrosis factor-α, C5a, lipopolysaccharide or IL-23 plus IL-1β did not affect IL-17A production in MCs.ConclusionsThe synovial MCs are not a main source of IL-17A in RA.



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