Activity and expression of Candida glabrata vacuolar proteases in autophagy-like conditions.

Activity and expression of Candida glabrata vacuolar proteases in autophagy-like conditions.

FEMS Yeast Res. 2018 Jan 29;:

Authors: Cortez-Sánchez JL, Cortés-Acosta E, Cueto-Hernández VM, Reyes-Maldonado E, Hernández-Rodríguez C, Villa-Tanaca L, Ibarra JA

Abstract
Candida glabrata is an emerging opportunistic pathogen that has intrinsic resistance to azoles antifungals. During infection or while living as a commensal it encounters nutritional stresses such as deficiency of carbon or nitrogen sources. Herein we investigate the expression and activity of PrA, Ape1, Ape3 and CpY vacuolar proteases during these stressful nutrimental conditions. Our findings demonstrate a differential activity profile depending on the addition or lack of carbon, nitrogen or both. Of the four proteases tested, PrA and Ape3 showed a higher activity in the absence of nitrogen. Steady-state RNA levels for all the proteases were also differentially expressed although not always correlated with its activity, suggesting multiple levels of regulation. Microscopy observations of C. glabrata cells subjected to the different conditions showed an increase in the vacuolar volume. Moreover, the presence of ATG8-PE and an increased expression of ATG8 were observed in the yeast under the tested conditions suggesting that C. glabrata is in autophagy stage. Taken together our results showed that PrA, Ape1, Ape3 and CpY have varying activities and expression depending on whether nitrogen or carbon is added to the media, and that these vacuolar proteases might have a role in the autophagy process.

PMID: 29385574 [PubMed - as supplied by publisher]

from #PM All via ola Kala on Inoreader http://ift.tt/2s0MdRb