TUC338 overexpression leads to enhanced proliferation and reduced apoptosis in tongue squamous cell carcinoma cells in vitro

Publication date: Available online 24 August 2016
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Ke-Xiong Ouyang, Rui Zou, Jun Liang, Zhi-bao Bai, Zhi-qiang Li, Jian-Jiang Zhao
PurposeLong non-coding RNAs (lncRNAs) are closely related to the development of tumors. In this study, we explored the contribution of the lncRNATUC338 to cellular processes in tongue squamous cell carcinoma(TSCC).MethodsFirst, we detected TUC338 expression using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in 25 patients. Then, we transfected a short hairpin RNA (shRNA) to silence TUC338 expression in the CAL-27 and SCC-9 cell lines. Tumor cell growth was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, and apoptosis and cell cycle analyses were performed via flow cytometry.ResultsThe results indicated thatTUC338 was overexpressed in TSCCs (P<0.05). Additionally, silencing TUC338 in CAL-27 and SCC-9 cells inhibited cell growth and increased apoptosis significantly in vivo(P<0.05).ConclusionsLncRNATUC338 overexpression leads to enhanced proliferation and reduced apoptosis in TSCC.



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