Tollip SNP rs5743899 Modulates Human Airway Epithelial Responses to Rhinovirus Infection

Abstract

Background

Rhinovirus (RV) infection in asthma induces varying degrees of airway inflammation (e.g., neutrophils), but the underlying mechanisms remain unclear.

Objective

The major goal was to determine the role of genetic variation (e.g., single nucleotide polymorphisms [SNPs]) of Toll-interacting protein (Tollip) in airway epithelial responses to RV in a type 2 cytokine milieu.

Methods

DNA from blood of asthmatic and normal subjects was genotyped for Tollip SNP rs5743899 AA, AG and GG genotypes. Human tracheobronchial epithelial (HTBE) cells from donors without lung disease were cultured to determine pro-inflammatory and anti-viral responses to IL-13 and RV16. Tollip knockout and wild-type mice were challenged with house dust mite (HDM) and infected with RV1B to determine lung inflammation and anti-viral response.

Results

Asthmatic subjects carrying the AG or GG genotype (AG/GG) compared with the AA genotype demonstrated greater airflow limitation. HTBE cells with AG/GG expressed less Tollip. Upon IL-13 and RV16 treatment, cells with AG/GG (versus AA) produced more IL-8 and expressed less anti-viral genes, which was coupled with increased NF-κB activity and decreased expression of LC3, a hallmark of the autophagic pathway. Tollip co-localized and interacted with LC3. Inhibition of autophagy decreased anti-viral genes in IL-13 and RV16 treated cells. Upon HDM and RV1B, Tollip knockout (versus wild-type) mice demonstrated higher levels of lung neutrophilic inflammation and viral load, but lower levels of anti-viral gene expression.

Conclusions and Clinical Relevance

Our data suggest that Tollip SNP rs5743899 may predict varying airway response to RV infection in asthma.

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