Comparison of the amount of bioaccessible fumonisin B 1 and B 2 in maize and rice inoculated with Fusarium verticillioides (MRC 826) and determined by in vitro digestion—preliminary results

Abstract

In this study the occurrence of hidden fumonisin B₁ (FB₁) and fumonisin B₂ (FB₂) was analysed, on two cereal substrates (maize and rice), inoculated with Fusarium verticillioides (MRC 826), in order to determine the ratio of hidden FB₁ and FB₂. Two parallel methods were applied: an in vitro human digestion sample pre-treatment and the routine extraction procedure, in both cases with subsequent LC-MS analysis. It was found that all samples showed higher concentration of total fumonisin B₁ after digestion, as compared to that of free fumonisin analysed only after extraction. The percentage of the hidden form by maize was 18.8 % (±2.4) for FB₁ and 36.8 % (±3.8) for FB₂, while for rice it was 32.3 % (±11.3) and 58.0 (±6.8), respectively, expressed as the proportion to total fumonisin B₁, for the total dataset. Significant differences were found in the FB₁ and FB₂ concentration measured after the different digestion phases (saliva, gastric and duodenal) in case of both matrixes. The results are useful for human risk assessment, since both humans and animals may be exposed to markedly higher toxin load, as determined merely by conventional analytical methods.

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