Association of T-regulatory cells and CD23/CD21 expression with vitamin D in children with asthma

Publication date: Available online 26 March 2016
Source:Annals of Allergy, Asthma & Immunology
Author(s): Anchoju Vijayendra Chary, Rajkumar Hemalatha, Machiraju Vasudeva Murali, Dodle Jayaprakash, Bharathraj Dinesh Kumar
BackgroundChildren with asthma have low vitamin D levels; however, we do not know whether low vitamin D is associated with impaired T-regulatory (Treg) cell population or high IgE receptors (CD23 and CD21) on B cells.ObjectivesTo examine Treg cell function, CD23 and CD21 receptors, vitamin D, and vitamin D–regulating enzymes in children with asthma.MethodsSixty children (2–6 years old) with asthma and 60 age-matched healthy children were selected as study participants. After collecting demographic and clinical data, blood samples were collected. Treg cells and CD23/CD21 expressions were evaluated by flow cytometry, 25-hydroxyvitamin D3 (25[OH]D3) was quantified by high-performance liquid chromatography, and cytokines and total IgE were estimated by enzyme-linked immunosorbent assay. Messenger RNA (mRNA) expressions of FOXP3, CD23, CD21, vitamin D receptors, and vitamin D–regulating enzymes were assessed by reverse transcription–polymerase chain reaction.ResultsThe 25(OH)D3 concentrations and proportion of Treg cells were lower (P < .05) among children with asthma. In contrast, the proportions of B cells with CD23 and CD21 expression were higher (P < .05) in patients with asthma compared with controls. Interleukin 10 and transforming growth factor β were also altered in asthma. The mRNA expression of CD23, CD21, and vitamin D receptors was up-regulated, whereas mRNA expression of vitamin D–regulating enzymes, CYP2R1, CYP27B1, and vitamin D binding protein (except CYP24A1) were up-regulated among children with asthma.ConclusionThe current study found impaired Treg cell population and high numbers of B cells with IgE receptors (CD23 and CD21) and altered regulatory cytokines in children with asthma, suggesting impaired immune regulation.



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