Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers?

xlomafota13 shared this article with you from Inoreader Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers? Via Journal of Otolaryngology - Head & Neck Surgery The malignancy potential of the laryngeal lesions are one of the major concerns of the surgeons about choosing the treatment options, forming surgical margins, deciding the follow-up periods. Finding a biomark... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Identification of E2F transcription factor 7 as a novel potential biomarker for oral squamous cell carcinoma

xlomafota13 shared this article with you from Inoreader Identification of E2F transcription factor 7 as a novel potential biomarker for oral squamous cell carcinoma Via Head & Face Medicine As a tumor-accelerating transcriptional factor, E2F transcription factor 7 (E2F7) was up-regulated in many forms of cancers. Nevertheless, little has been reported about the impacts of E2F7 on oral squamous ce... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Preparation and validation of cyclodextrin-based excipients for radioiodinated hypericin applied in a targeted cancer radiotherapy

xlomafota13 shared this article with you from Inoreader Preparation and validation of cyclodextrin-based excipients for radioiodinated hypericin applied in a targeted cancer radiotherapy Via radioactive iodine Int J Pharm. 2021 Feb 24:120393. doi: 10.1016/j.ijpharm.2021.120393. Online ahead of print. ABSTRACT BACKGROUND: Iodine-131 labeled hypericin (131I-Hyp) has been utilized as a necrosis-avid theragnostic tracer in a dual targeting pan-anticancer strategy called OncoCiDia. Widespread use of previously-tested solvent dimethyl sulfoxide (DMSO) is limited by safety concerns. To tackle this, the present study was designed to explore a clinically feasible excipient for the form ulation of the hydrophobic 131I-Hyp for intravenous administration. METHOD: Solubility of Hyp in serial solutions of already-approved hydroxypropyl-β-cyclodextrin (HP-β-CD) was evaluated by UVspectrophotometry and 50% HP-β-CD was chosen for further experiments. Two novel HP-β-CD-based formulations of 131I-Hyp were compared with previous DMSO-based formulation, with regards to necrosis-targetability and biodistribution, by magnetic resonance imaging, single-photon emission computed tomography (SPECT), gamma counting, autoradiography, fluorescence microscopy and histopathology. RESULTS: Hyp solubility was enhanced with increasing HP-β-CD concentrations. The radiochemical purity of 131I-Hyp was higher than 90% in all formulations. The necrosis-targetability of 131I-Hyp in the novel formulations was confirmed in vivo by SPECT and in vitro by autoradiography, fluorescence microscopy and histopathology. The plasma clearance of r adioactivity was faster in the novel formulations. CONCLUSION: The novel 131I-Hyp formulations with HP-β-CD could be a suitable pharmaceutical excipient for 131I-Hyp for intravenous administration. PMID:33639227 | DOI:10.1016/j.ijpharm.2021.120393 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Remote medulla ablongata ventral acute subarachnoid hemorrhage following cervical spinal surgery: A case report

xlomafota13 shared this article with you from Inoreader Remote medulla ablongata ventral acute subarachnoid hemorrhage following cervical spinal surgery: A case report Via leak of cerebrospinal fluid Int J Surg Case Rep. 2021 Feb 22;80:105675. doi: 10.1016/j.ijscr.2021.105675. Online ahead of print. ABSTRACT INTRODUCTION: The incidence of remote intracranial hemorrhage (RICH) in patients during spinal surgery is rare and the detailed mechanism remains unclear. PRESENTATION OF CASE: A 55-year-old man had undergone cervical discectomy and fusion at C5-6 and C6-7 due to herniated disc and secondary spinal canal stenosis. He had severe headache 20 h postoperatively and his drain output increased from 100 to 350 mL in the second 10 h after surgery. Computed tomography (CT) and magnetic resonance imaging (MRI) were performed and he was diagnosed with acute subarachnoid hemorrhage in the ventral medulla oblongata. The drainage tube was quickly removed. Infusion of hypertonic saline was used to reduce intracranial pressure and nimodipine prevented vasospasm around the brainstem. The patient made a gradual, satisfactory recovery with conserv ative treatment. DISCUSSION: The most likely pathomechanism leading to RICH is venous bleeding due to rapid leak of a large amount of cerebral spinal fluid (CSF) after spinal surgery. If the patient has a headache or neurological complaints after spinal surgery, immediate imaging is recommended to confirm the diagnosis. Treatment depends on the amount and location of intracranial hemorrhage. CONCLUSION: RICH is a serious but rare complication of spinal surgery and cerebellar hemorrhage is the most common. The most important pathomechanism leading to RICH after spinal surgery is venous bleeding due to rapid leak of a large amount of CSF. Timely CT is necessary to exclude RICH. Treatment of RICH depends on the size of the intracranial hematoma and the patient's symptoms. PMID:33639502 | DOI:10.1016/j.ijscr.2021.105675 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL‐36γ and IL‐37

xlomafota13 shared this article with you from Inoreader Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL‐36γ and IL‐37 Via Experimental Dermatology Abstract Phototherapy with narrow‐band Ultraviolet B (nb‐UVB) is a major therapeutic option in atopic dermatitis (AD), yet knowledge of the early molecular responses to this treatment is lacking. The objective of this study was to map the early transcriptional changes in AD skin in response to nb‐UVB treatment. Adult patients (n = 16) with AD were included in the study and scored with validated scoring tools. AD skin was irradiated with local nb‐UVB on day 0, 2 and 4. Skin biopsies were taken before and after treatment (day 0 and 7) and analysed for genome‐wide modulation of transcription. When examining the early response after three local UVB treatments, gene expression analysis revealed 77 significantly modulated transcripts (30 down‐ and 47 upregulated). Among them were transcripts related to the inflammatory response, melanin synthesis, keratinization and epidermal structure. Interestingly, the pro‐inflammatory cytokine IL‐36γ was reduced after treatm ent, while the anti‐inflammatory cytokine IL‐37 increased after treatment with nb‐UVB. There was also a modulation of several other mediators involved in inflammation, among them defensins and S100 proteins. This is the first study of early transcriptomic changes in AD skin in response to nb‐UVB. We reveal robust modulation of a small group of inflammatory and anti‐inflammatory targets, including the IL‐1 family members IL36γ and IL‐37, which is evident before any detectable changes in skin morphology or immune cell infiltrates. These findings provide important clues to the molecular mechanisms behind the treatment response and shed light on new potential treatment targets. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A Systematic Review of the Omohyoid Muscle Syndrome (OMS): Clinical Presentation, Diagnosis, and Treatment Options

xlomafota13 shared this article with you from Inoreader A Systematic Review of the Omohyoid Muscle Syndrome (OMS): Clinical Presentation, Diagnosis, and Treatment Options Via otol rhinol Ann Otol Rhinol Laryngol. 2021 Feb 25:3489421995037. doi: 10.1177/0003489421995037. Online ahead of print. ABSTRACT OBJECTIVE: Omohyoid muscle syndrome (OMS) is a condition that causes a X-shaped lateral neck lump on swallowing, caused by the failure of the central tendon of the omohyoid muscle to restrict movement of the muscle during swallowing. We aim to review the etiology, pathophysiology, diagnostic tests, and management options for this condition. DATA SOURCES: Pubmed, MEDLINE, EMBASE, and Cochrane databases were searched for all articles and abstracts related to OMS up to 29th July 2020. REVIEW METHODS: A systematic review was performed, data extracted from relevant full text articles. Both quantitative data and qualitative data were analyzed. RESULTS: Twenty cases of OMS were reported. Patients presented at a mean age of 36.0. All cases were Asian. There is a 7:3 ratio of males to females. The most common symptom was a transient neck mass. Most cases were managed conservatively with good prognosis. Open or endoscopic transection of the muscle and ultrasound-guided botulinum toxin injection were 3 treatment options, with no recurrence at 4 years, 6 months, and 6 months respectively. CONCLUSION: OMS could be genetic as all cases were Asian in ethnicity. The deep cervical fascia which usually envelopes the omohyoid muscle may be weakened by stress as 20% of cases had a preceding traumatic event. Real-time ultrasonography e stablishes the diagnosis, demonstrating the anterolateral displacement of the sternocleidomastoid muscle by a thickened omohyoid muscle during swallowing. Surgical transection can achieve cure, but due to limited studies available, they should be reserved for patients who are extremely bothered. Intramuscular injection of botulinum toxin is an effective alternative, but recurrence is expected. PMID:33626894 | DOI:10.1177/0003489421995037 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Tympanostomy Tubes-Where We Were, Where We Are, and Where We Will Be: A State-of-The-Art Review

xlomafota13 shared this article with you from Inoreader Tympanostomy Tubes-Where We Were, Where We Are, and Where We Will Be: A State-of-The-Art Review Via ear nose throat Ear Nose Throat J. 2021 Feb 24:145561321997612. doi: 10.1177/0145561321997612. Online ahead of print. NO ABSTRACT PMID:33626919 | DOI:10.1177/0145561321997612 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Combined treatment of ureteropelvic junction obstruction and renal calculi with Robot Assisted Laparoscopic Pyeloplasty and Laser Lithotripsy in children: case report and non systematic review of the literature

xlomafota13 shared this article with you from Inoreader Combined treatment of ureteropelvic junction obstruction and renal calculi with Robot Assisted Laparoscopic Pyeloplasty and Laser Lithotripsy in children: case report and non systematic review of the literature Via The International Journal of Medical Robotics and Computer Assisted Surgery Summary: Objective(s) The incidence of urinary tract stone disease is steadily increasing in both adult and paediatric populations. This condition develops due to different factors: dietary or metabolic alterations, infection and congenital anatomic malformations. Standard indications and treatments for children are analogous to the ones indicated for adults. Extracorporeal shock wave lithotripsy (SWL), ureterorenoscopy (URS), percutaneous nephrolithotomy (PCNL) should be preferred to more invasive techniques. Moreover, the introduction of laparoscopic and robot assisted laparoscopic approaches have improved surgical outcomes, lowering the bleeding risk with higher stone‐free rates, even in complicated cases. Despite these well‐known improvements, there are few reports regarding laparoscopic robot assisted management for urinary tract stone disease in paediatric patients under the age of ten, especially with concomitant treatment of ureteropelvic junction obstruction and multiple calyceal stones. Patient and Method(s) A four years old child was referred for recurrent right abdominal flank pain, macroscopic haematuria and a previous history of urinary tract infections. A computed tomography of the abdomen showed right ureteropelvic junction obstruction associated with multiple unilateral stones located in the renal pelvis and in the interpolar renal calyces. Due to its complexity, we held a multidisciplinary meeting with paediatric surgeons and nephrologists to determine optimal treatment. As a result, a combined robot assisted laparoscopic pyeloplasty and renal calculi holmium laser lithotripsy using a digital flexible ureteroscope through an abdominal robotic trocar was performed. No post‐surgical complications were recorded and the patient was discharged within 48 hours following surgery. At subsequent regular follow‐up examinations over a period of 24 months, no signs of recurrence were detected for both ureteropelvic junction obstruction and stone disease. Result(s) Robot Assisted Laparoscopic Pyeloplasty with concomitant laser lithotripsy is a reasonable treatment option for designated young paediatric patients with challenging ureteropelvic junction obstruction complicated by urolithiasis, especially in cases where stones are not amenable with standard procedures. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Association Between Habitual Snoring and Cognitive Performance in Preadolescent Children

xlomafota13 shared this article with you from Inoreader Association Between Habitual Snoring and Cognitive Performance in Preadolescent Children Via JAMA Otolaryngology–Head & Neck Surgery Online First This cross-sectional study uses a baseline data set from the on going Adolescent Brain and Cognitive Development study to examine the extent to which potential confounding factors modify the association between parent-reported habitual snoring and cognitive outcomes among preadolescent children. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Otolaryngologic Manifestations in Pediatric Inflammatory Multisystem Syndrome Temporally Associated With COVID-19

xlomafota13 shared this article with you from Inoreader Otolaryngologic Manifestations in Pediatric Inflammatory Multisystem Syndrome Temporally Associated With COVID-19 Via JAMA Otolaryngology–Head & Neck Surgery Online First This cohort study describes the various otolaryngologic manifes tations in and rates among patients 18 years or younger with pediatric inflammatory multisystem syndrome temporally associated with severe acute respiratory syndrome coronavirus 2. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Laryngeal and Tracheal Pressure Injuries in Patients With COVID-19

xlomafota13 shared this article with you from Inoreader Laryngeal and Tracheal Pressure Injuries in Patients With COVID-19 Via JAMA Otolaryngology–Head & Neck Surgery Online First To the Editor The important study by Fiacchini and colleagues of laryngotracheal complications in ventilated patients with coronavirus disease 2019 (COVID-19) reported a staggering 47% incidence of full-thickness tracheal erosion (FTTE) and tracheoesophageal fistulae (TEF). These findings raise critical questions about ensuring safe, high-quality care for intubated patients. Although intense research has focused on the molecular basis of COVID-19, pathogenesis of pneumonitis, and novel therapeutics; far less work has addressed the prevalence and pathogenesis of iatrogenic injury—particularly with respect to airway device-related pressure injuries. Injury at the level of the tracheal cuff likely accounts for the preponderance of FTTE and TEF reported, and tissue injury may occur at any site where there is contact between a device and the aerodigestive tract. Risk is highest in patients with overzealous cuff inflation, high-dose steroids, prone ventilation, prolonged intubation, feeding tubes, or impaired wound healing from diabetes or radiation—a perfect storm for patients with COVID-19. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Prematurity: A Prognostic Factor for Increased Severity of Pediatric Obstructive Sleep Apnea

xlomafota13 shared this article with you from Inoreader Prematurity: A Prognostic Factor for Increased Severity of Pediatric Obstructive Sleep Apnea Via The Laryngoscope Objective/Hypothesis Studies have suggested preterm birth, defined as gestational age (GA) <37 weeks, is a risk factor for obstructive sleep apnea (OSA) in later childhood. However, little is known about the characteristics, severity, and degree of intervention of childhood OSA in former preterm infants compared to term infants. This study compares polysomnographic characteristics and surgical interventions in former preterm and term infants presenting with sleep disordered breathing. Study Design Retrospective cohort study from 2015 to 2019 at a single tertiary referral center. Methods Electronic Medical Records of pediatric patients ages 0 to 18 presenting with sleep disordered breathing were reviewed for gestational age, polysomnographic findings, clinical characteristics, and OSA surgical interventions. Association between gestational age, polysomnographic characteristics, and surgical interventions for OSA were reported. Results A total of 615 patient records were analyzed. Adjusting for covariates, prematurity was associated with a 2.97× higher likelihood of development of severe OSA (aOR (95%CI): 2.97 (1.40–6.32)), increased apneic‐hypoxic index (AHI) (mean (SD): 6.5 (9.8) vs. 4.6 (6.4), P < .05), increased end tidal CO2 (50.5 (5.11) vs. 48.5 (5.8), P < .05), decreased REM latency (116 (64.7) vs. 132.4 (69.9), P < .05), and increased number of surgeries for OSA (0.65 (.95) vs. 0.45 (0.69), P < .05) compared to children born at term. Children born with GA < 32 weeks presented at a significantly later age with sleep disordered breathing (7.04 (.80) vs. 5.1 (0.15), P < .05) than children born at term. Conclusions Prematurity was associated with increased likelihood of severe OSA, increased AHI, as well as increased number of surgical interventions for OSA compared to children born at term. These results suggest an association with preterm birth and increased severity of childhood OSA. Level of Evidence III Laryngoscope, 2021 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.